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Sorting
If you are planning on sorting sterile cells, you need to have prepared
your cells completely at your facility. That means having them in the correct
tubes (Falcon 2063’s), filtered and at the correct concentration (Approx.
60-70 x 10^6 cells/ml.). Cells can be transferred and filtered at the facility, but we make no
guarantees of sterility, as we do not have access to a sterile room.
Please remember to bring with you: collection tubes, collection media,
ice bucket (or something to hold the collection tubes in) and a PC
formatted zip disk.
Controls required for your sort should include a negative, or unstained
control, and single-stained positive control for each fluorochrome you are
using.
If you are sorting into anything other than a tube, (96 well plate for
example) please bring the plates preloaded with media, and an extra empty plate
for instrument calibration.
If you are planning on sticking around during the sort, you may want to
bring a book, or something to work on.
Flow Cytometry Acquisition/Analysis
Requirements
If you are doing FACS analysis, here are some things you may need to
bring with you.
MAC formatted 250MB Zip disk. Data is backed up and archived, but
we are not responsible for inadvertently lost data
Pipet and tips.
**If you are doing PI staining for cell cycle, please make sure you have
filtered your cells. Cells stained with PI tend to clump and will clog the
FACScan.
FACS Facility Rules
We don’t like having a lot of unnecessary rules to complicate things,
so please follow these simple rules:
1. CLEAN UP AFTER YOURSELF. Anything left in the facility will become the
property of the facility, or thrown away.
2. CLEAN UP AFTER YOURSELF. To keep the instrument running consistently,
please flush the system with bleach and water after your run.
3. CLEAN UP AFTER YOURSELF. Please either take your trash with you or throw
it away. There are trashcans in the lab, and they aren’t located on the
benches.
4. CLEAN UP AFTER YOURSELF. Actually that should read Respect Others
Appointments, but I had a pattern going, and I didn’t want to break it. If
someone is signed up after you, they have the right to ask you to finish up and
stop. Remember, by delaying them, they may delay the next person, etc. etc.
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