Beth Israel Deaconess Medical Center 330 Brookline Ave; HM-953
Boston, MA 02215
Advanced Degree And Training Info:
Year
Institution
Area or Rank
No data available
Research Team Listing
No team members listed
Areas of Interest:
Cancer & Hematologic
Major Research Theme:
My postdoctoral training took place in the laboratory of Dr. Daniel Tenen at Beth Israel Deaconess Medical Center, where I was engaged in a number of projects related to normal myeloid cell development and molecular causes of leukemia. In one project, aimed to investigate regulation of the human CD34 gene, I found that the crucial cis element(s) are located in a remote position from the gene and efforts are currently under way to map them. In a second project, I turned my attention to the role of CCAAT/Enhancer Binding Protein (C/EBP) family members in myeloid cell differentiation. These studies identified C/EBPalpha transcription factor as a molecular switch of granulocytic differentiation. Since C/EBPalpha function and/or expression was found to be perturbed in many cases of AML patients, I designed a screen of a chemical library to find the compounds that upregulate C/EBPalpha expression, or increase its transcriptional activity, and may potentially give rise to new therapeutics for treatment of AML. Finally, I am interested in modulation of C/EBPalpha function by phosphorylation, as a response to activation of various signaling pathways during leukemogenesis, and identifying protein partners of C/EBPalpha modulating its activity in phosphorylation-dependent manner.
Radomska HS, Basseres DS, Zheng R, Zhang P, Dayaram T, Yamamoto Y, Sternberg DW, Lokker N, Giese NA, Bohlander SK, Schnittger S, Delmotte MH, Davis RJ, Small D, Hiddemann W, Gilliland DG, Tenen DG. “Block of C/EBPa function by phosphorylation in acute myeloid leukemia with FLT3 activating mutations”, J Exp Med. 2006; 203(2):371-81.
Ross SE*, Radomska HS*, Wu B, Zhang P, Winnay JN, Bajnok L, Wright WS, Schaufele F, Tenen DG, Mac Dougald, OA. “Phosphorylation of C/EBPalpha inhibits granulopoiesis”, Mol Cell Biol. 2004; 24:675-686; *equal contribution.
Radomska HS*, Gonzalez DA, Okuno Y, Iwasaki H, Nagy A, Akashi K, Tenen DG, Huettner CS. “Transgenic targeting with regulatory elements of the human CD34 gene”, Blood 2002; 100:4410-9; *corresponding author.
Radomska HS, Huettner CS, Cheng T, Scadden DT, Tenen DG. CCAAT/Enhancer Binding Protein α Is a Regulatory Switch Sufficient For Induction of Granulocytic Development From Bipotential Myeloid Progenitors, MCB 1998; 18:4301-4314.
Radomska HS, Satterthwaite AB, Burn TC, Oliff IA, Huettner CS, Tenen DG. Multiple Control Elements Are Required For Expression of the Human CD34 Gene, Gene 1998; 222:305-318.
External Recognition:
Harvard Medical School, Scholar in Medicine Award, 2000-2001
NIH, Mentored Research Scientist Development Award, K 01 (DK62064); 2002-2005
